Review



primary antibody incubation  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Thermo Fisher primary antibody incubation
    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of <t>incubation</t> with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Primary Antibody Incubation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/TBS+with+Tween+(TBST)%2C+Solution%2C+Molecular+Biology+Grade%2C+Ultrapure/bio_rxiv__64898__2026__04__01__715918-194-30-23
    Average 97 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    97/100 stars

    Images

    1) Product Images from "Neural cell state modulation by PARK2 and dopaminergic neuroprotection by small molecule Parkin agonism"

    Article Title: Neural cell state modulation by PARK2 and dopaminergic neuroprotection by small molecule Parkin agonism

    Journal: bioRxiv

    doi: 10.64898/2026.04.01.715918

    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Figure Legend Snippet: ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Techniques Used: Staining, Derivative Assay, Imaging, Incubation, Control, Western Blot, Transmission Assay, Electron Microscopy, Recombinant, Sonication



    Similar Products

    primary antibody incubation  (Developmental Studies Hybridoma Bank)
    96
    Developmental Studies Hybridoma Bank primary antibody incubation
    Primary Antibody Incubation, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/anti-vasa/pmc13008353-234-12-19
    Average 96 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Vector Laboratories)
    96
    Vector Laboratories primary antibody incubation
    Primary Antibody Incubation, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/pmc13076796-218-13-5
    Average 96 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Thermo Fisher)
    97
    Thermo Fisher primary antibody incubation
    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of <t>incubation</t> with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Primary Antibody Incubation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/TBS+with+Tween+(TBST)%2C+Solution%2C+Molecular+Biology+Grade%2C+Ultrapure/bio_rxiv__64898__2026__04__01__715918-194-30-23
    Average 97 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    97/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc primary antibody incubation
    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of <t>incubation</t> with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Primary Antibody Incubation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/pmc13068634-311-0-11
    Average 86 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Servicebio Inc)
    86
    Servicebio Inc primary antibody incubation
    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of <t>incubation</t> with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Primary Antibody Incubation, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/1+anti+il+%CE%B2/pm41928263-76-15-20
    Average 86 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Huabio Inc)
    86
    Huabio Inc primary antibody incubation
    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of <t>incubation</t> with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.
    Primary Antibody Incubation, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/antibody+incubation+primary/pm41895394-223-2-14
    Average 86 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    primary antibody incubation against tnf α  (Proteintech)
    96
    Proteintech primary antibody incubation against tnf α
    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis <t>of</t> <t>TNF-α</t> protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Primary Antibody Incubation Against Tnf α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/TNF+alpha+Antibody/pmc13034476-96-0-5
    Average 96 stars, based on 1 article reviews
    primary antibody incubation against tnf α - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier
    primary antibody incubation  (Biorbyt)
    96
    Biorbyt primary antibody incubation
    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis <t>of</t> <t>TNF-α</t> protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Primary Antibody Incubation, supplied by Biorbyt, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/antibody/pm41826998-136-0-10
    Average 96 stars, based on 1 article reviews
    primary antibody incubation - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Journal: bioRxiv

    Article Title: Neural cell state modulation by PARK2 and dopaminergic neuroprotection by small molecule Parkin agonism

    doi: 10.64898/2026.04.01.715918

    Figure Lengend Snippet: ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Article Snippet: Membranes were incubated with primary antibodies overnight at 4°C following blocking with 5% Bovine Serum Albumin (Millipore Sigma, A9647-100G) in TBS with Tween (Thermo Scientific Chemicals, J77500.K2) for 1 h. Primary antibody incubation was done overnight, while secondary antibody incubation was done at room temperature for 1 h. Membranes were imaged using ChemiDoc (Bio-Rad) and analyzed using Image Lab software (Bio-Rad).

    Techniques: Staining, Derivative Assay, Imaging, Incubation, Control, Western Blot, Transmission Assay, Electron Microscopy, Recombinant, Sonication

    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Nutrition

    Article Title: Lactobacillus acidophilus alleviates slow transit constipation by modulating 5-HT pathway and gut microbial composition

    doi: 10.3389/fnut.2026.1775405

    Figure Lengend Snippet: Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Primary antibody incubation against TNF-α (Proteintech, Wuhan, China) (60291-1-IG) (dilution 1:1000) was performed overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Quantitative RT-PCR